Cell-attached voltage-clamp and current-clamp recording and stimulation techniques in brain slices
نویسندگان
چکیده
منابع مشابه
One-channel cell-attached patch-clamp recording.
Ion channel proteins are universal devices for fast communication across biological membranes. The temporal signature of the ionic flux they generate depends on properties intrinsic to each channel protein as well as the mechanism by which it is generated and controlled and represents an important area of current research. Information about the operational dynamics of ion channel proteins can b...
متن کاملVoltage clamp techniques
Much of what we know about the properties of ion channels in cell membranes has come from experiments using voltage clamp. In general, the method allows ion flow across a cell membrane to be measured as electric current, whilst the membrane voltage is held under experimental control with a feedback amplifier. The method was first developed by Cole (1949) and Hodgkin et al. (1952) for use with t...
متن کاملThe interpretation of current-clamp recordings in the cell-attached patch-clamp configuration.
In these experiments we have investigated the feasibility and accuracy of recording steady-state and dynamic changes in transmembrane potential noninvasively across an intact cell-attached patch using the current-clamp mode of a conventional patch-clamp amplifier. Using an equivalent circuit mimicking simultaneous whole-cell voltage-clamp and cell-attached current-clamp recordings we have defin...
متن کاملPatch clamp techniques for single channel and whole-cell recording
The patch clamp technique was first used by Neher and Sakmann (1976) to resolve currents through single acetylcholine-activated channels in cell-attached patches of membrane of frog skeletal muscle. The method they used (described by Neher, Sakmann & Steinbach, 1978) and subsequent refinements (Hamill, Marty, Neher, Sakmann & Sigworth, 1981) have led to techniques for high resolution recording ...
متن کاملthe effect of quasitropezoidal pulses on l-type ca2+ current of somata of f1 neuron in helix aspersa using intracellular recording techniques (voltage and current clamp)
introduction: the ionic currents show nonlinear behavior and electrical stimuli can influence their activation and inactivation kinetics. the time constant of depolarizing or hyperpolarizing current pulses has an important role in the firing behavior of neurons. in the present study, the effects of quasitrpezoidal command potentials on l-type calcium channel of somata of f1 neuron in helix aspe...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 2006
ISSN: 0165-0270
DOI: 10.1016/j.jneumeth.2006.02.010